Experimental Design
 

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 Experimental design
The experiment is currently scheduled to run 5 years (2006-2010). The experiment will utilize Free Air CO2 Enrichment (FACE) technology designed by IBIMET, and infra-red heaters installed in 3.4 m diameter rings to expose the native NMP and also the NMP inter-planted to perennial weeds (below) to four combinations of CO2 and temperature, plus two watering treatments.  The two water treatments are designed to 1) simulate the effect of CO2 on plant water use efficiency with water additions, thereby evaluating how much of the CO2 responses were due simply to improved plant/soil water relations, and 2) separate the desiccating effect of infra-red warming from the purely temperature responses. Each of the six treatments will be replicated in five rings, for a total of 30 rings (map).   The treatments are designated as follows:

ACN - ambient CO2, control temp, non-irrigated

AHN - ambient CO2, infra-red heating, non-irrigated

ECN - elevated CO2 (570 µL L-1 CO2), control temp, non-irrigated

EHN - elevated CO2, infra-red heating, non-irrigated

ACI - ambient CO2, control temp, irrigated (to simulate CO2 water savings)

AHI - ambient CO2, infra-red heating, irrigated (to evaluate water-temp responses)

 

Each of the rings will be split into two sections by a 25 cm steel flange (ring diagram flange is oriented with prevailing wind).  One side features a northern mixed prairie (NMP) partitioned into soil and plant sampling areas, plus sections for trace gas measurements, a minirhizotron tube (Bartz Technology) for studying root responses, soil water content (Sentek Envirosmart sensors) and air and soil temperature measurements.  The  area on the other side of the ring is partitioned into a one m2 interplanting of Linaria dalmatica (Dalmation Toadflax) and 8 0.124 m2 areas with winter annual grasses (Bromus tectorum, Vulpia octiflora), annual mustards (Descurainia pinnata, D. Sophia) annual lambsquarters (Chenopodium album, C. leptophyllum) and biennial composites (Centaurea diffusa, Grindelia squarosa.  The weed vs. NMP areas are randomly assigned within each ring, and the weed species are randomly assigned to weed zones.  An approximate 20 cm wide buffer strip prevents sampling at the outer circle edge where accurate CO2 control in the elevated CO2 rings is not possible and where plant-mediated edge effects prevail.

 

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Last modified: 09/28/06